樟树内生细菌EBS05发酵条件的研究

Fermentation Conditions of Endophytic Bacteria Strain EBS05 Isolated from Camphor Tree(Cinamonum camphora)

枯草芽胞杆菌EBS05是从樟树中分离的1株对多种植物病原真菌具有拮抗作用的内生细菌。以小麦纹枯病菌为靶标菌。通过单因素试验和正交设计试验对其发酵条件进行了优化。结果表明，内生细菌EBS05适宜的发酵培养基主要营养成分的组成和配比分别为可溶性淀粉3％、蛋白胨2％、NaCl0．25％。最佳发酵条件为：初始pH5—9，最适温度34℃，装液量25mlM250mL三角瓶，接种量3％，发酵时间72h。

Bacillus subtills EBS05 is an endophytic bacterium strain isolated from camphor tree （Cinamonam camphora） possessing antagonistic role against many plant pathogenic fungi. Its fermentation conditions were optimized by means of one-factor design and orthogonal design experiment methods using Rhizoctonia cerealis as a target pathogen. The results indicated that the optimum fermentation medium was respectively constituted and proportioned of soluble starch 3% , peptone 2% and sodium chloride 0.25%. The optimum fermentation conditions were as following： initial pH at 5 ~ 9, temperature at 34 ℃ , medium filling volume 25 mL/250 mL flask, inoculation volume 3% , fermented for 72 h.