高原人附红细胞体电镜形态特点

Observation of Human Eperythrozoon Ultrastructure At High Altitude

目的为进一步认识高原人附红细胞体病,对高原人附红细胞体超微结构进行研究。方法取海拔3700米确诊附红细胞体病患者血液或骨髓液进行电镜观察。结果扫描电镜观察下可见附红细胞体为大小不一的多形体小体,附红细胞体寄生在红绌胞表面,并不进入红细胞内;透射电镜观察附红细胞体大小不等,以球形为主的多形性小体,直径多为0.2～0.9μm最大可达1.5μm,偶见短杆状,半月形等不规则小体;当附红细胞体寄生在表面时,将其附着的两侧膜蛋白排挤呈聚集状,打破了原来排列整齐的状态,其外仅有一层膜包裹,此处膜形成洞,红细胞体积较其他红细胞大些。结论高原环境条件附红细胞体的电镜观察,为确诊附红细胞体的隐性感染和鉴别诊断提供依据。

Objective To further understand human Eperythrozoonosis of high altitude on the plateau, the Eperythrozoon ultrastructure is been observed. Methods The blood or bone marrow from diagnosed epreythrozoonsis patients is observed under electron microscopy at 3. 700 meters altitude. Results By scaHning of electron microscope, we Call see the multi-shaped small body of eperythrozoon, attached to ervthrocyte surface, not into cyton region. Transmission eleetlon microscopy showes eperythrozoon＇ s sizes to spherical-based polymorphism corpuscles, mostly in diameter 0.2 -0.9 μm. up to 1.5 μm. occasionally short rod, haft - moon and other irregular Corpuscles, When Eperythrozoon is parasitic on erythrocyte surface, it change its membrane proteins to together form, forming a erythrocyte membrane channel near attachment area. Conclusion The electron microscope results can provide basis for the differential diagnosis and recessive infection of Eperythrozoonosis at high altitude.