摘要
目的:探讨血管紧张素Ⅱ(angiotensinⅡ,AngⅡ)对体外人胰岛细胞内葡萄糖转运蛋白2(GLUT-2)mRNA的影响,通过体外实验了解血管紧张素Ⅱ影响胰岛细胞分泌功能的相关分子机制。方法:人胰岛细胞被分成空白对照组,AngⅡ组(100 nmol/L)和氯沙坦组(1μmol/L),以半定量RT-PCR检测细胞内GLUT-2mRNA的表达情况。结果:在100nmol/L AngⅡ作用下,人胰岛细胞GLUT-2 mRNA的表达显著低下(P<0.05);AngⅡ的这种作用,可被氯沙坦部分逆转。结论:AngⅡ可能通过抑制GLUT-2的表达而影响胰岛β细胞胰岛素的分泌。
Objective：To investigate effects of angiotensin Ⅱ on GLUT-2 mRNA in human islet βcells in vitro.To evaluate the role of GLUT-2 signaling pathway in Ang Ⅱ-mediated β-cell dysfunction.Methods：Human islet cells were divided into control group,Ang Ⅱ group（100nmol/L）,Losartan group（1μmol/L）Semi-quantitative RT-PCR were used to detected GLUT-2 mRNA expression.Results：GLUT-2 mRNA expression of human islet cells were significantly lower（P〈0.05） under the action of 100 nmol/L Ang Ⅱ.But losartan can partly reverse these effects.Conclusion：Ang Ⅱ may inhibit GLUT-2 expression,as a result β-cell insulin secretion decreased.
出处
《内蒙古医学院学报》
2010年第4期350-353,共4页
Acta Academiae Medicinae Neimongol
基金
国家自然科学基金(30470829)
