紫草提取物对小鼠急性肝损伤保护作用的研究

Protective Effect of Radix Lithospermi Extract Against Acute Liver Injury in Mice

目的:研究紫草提取物对四氯化碳所致小鼠急性肝损伤的保护作用,明确紫草保肝作用主要有效成分及其含量。方法:采用小鼠CCl4急性肝损伤模型,以血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)的活性,肝组织中还原性谷胱甘肽(GSH)活性、丙二醛(MDA)含量和肝组织形态学变化为指标,观察紫草提取物对肝损伤的保护作用;利用HPLC测定提取物中萘醌化合物含量。结果:紫草各剂量组(1.2、2.4、4.8g/kg)不同程度降低小鼠血清AST、ALT活力(P<0.01),显著降低MDA含量(P<0.5),减轻肝损伤程度,但对GSH活性没有显著影响。提取物中主要成分为萘醌类,其总含量为65.44mg/100mg。结论:紫草提取物对四氯化碳所致小鼠急性肝损伤具有保护作用,主要有效成分为紫草萘醌类化合物。

Objective：To study the protective effect of Radix Lithospermi extract against acute liver injury induced by carbon tetrachloride in mice and determine the major active pigments in Radix Lithospermi extract by HPLC.Methods：The animal model of acute liver injury was established by intraperitoneal injection of 0.2% carbon tetrachloride olive oil 10 ml/kg,the alanine aminotransferase （ALT）,aspartate aminotransferase （AST） in serum and malondialdehyde （MDA）,reduced glutathione hormone （GSH） in liver homogenate,the histopathological changes of the liver were assayed. The contents of naphthaquinones in Radix Lithospermi extract was determined by HPLC.Results：At all doses,Radix Lithospermi extract decreaced activities of serum ALT and AST. Radix Lithospermi extract significantly reduced the content of MDA and improved liver injuries; but it had no effect on activity of GSH.Conclusion：Radix Lithospermi extract showed protective effect against acute liver injury induced by carbon tetrachloride in mice and the major active pigments were naphthaquinones in Radix Lithospermi.