吗啡依赖大鼠胰岛A细胞免疫组织化学研究

Immunohistochemical study of A-cell of pancreatic islets inmorphine dependent rats

目的研究吗啡依赖大鼠Ａ细胞的变化，为探讨吗啡对Ａ细胞分泌功能的影响提供形态学依据。方法用皮下注射吗啡建立吗啡依赖大鼠模型，用免疫组织化学和图象分析方法观察胰岛Ａ细胞的变化。结果吗啡依赖大鼠Ａ细胞免疫反应增强（Ｐ＜０．０１）。结论吗啡可引起胰岛Ａ细胞分泌胰高血糖素的功能增强。

Objective To observe changes of A-cell of pancreatic islets and to put forward the morphological evidence to further research on the effects of morphine on the secretion of A-cell of pancreatic islets.Method The morphine dependent model of big rat was made by subcutaneous injection administrating morphine and observed by immunohistochemical survey and image analysis.Results Immumoreaction increased in the A-cell of pancreatic islets of the morphine dependent of big rat.Conclusion The morphine can give rise to increase in the function of the glucagon secreted by the A-cell of pancreatic islets.