非免疫缺陷相关性B细胞淋巴瘤与EB病毒的相关性研究

INVESTIGATION OF NON IMMUNODEFICIENCY RELATED B CELL LYMPHOMA ASSOCIATED WITH EPSTEIN BARR VIRUS INFECTIONGuo Xiaohong,Luo Rong,Gao Zifen, et al

目的探讨非免疫缺陷相关性淋巴结内Ｂ细胞淋巴瘤与ＥＢ病毒感染的关系。方法常规染色进行组织学分类，利用免疫组织化学ＬＳＡＢ法确定肿瘤的免疫表型。采用原位杂交方法检测ＥＢ病毒编码的小核ＲＮＡ（ＥＢＥＲｓ）。结果４０例淋巴结内Ｂ细胞淋巴瘤中，低度恶性２０例（中心母细胞－中心细胞性１２例、中心细胞性４例、淋巴细胞性４例），高度恶性２０例（中心母细胞性１７例、免疫母细胞性２例、Ｂｕｒｋｉｔｔ样１例）。所有病例中，Ｅ－ＢＥＲｓ阳性３例，占所检病例的７．５％，其中低度恶性１例，占低度恶性病例的５％，高度恶性２例，占高度恶性病例的１０％，阳性细胞约占肿瘤细胞的３０％～６０％。结论非免疫缺陷相关性淋巴结内Ｂ细胞淋巴瘤可能与ＥＢ病毒的感染有关，但不是主要致病因素。感染率与恶性程度无明显关系。

Objective To Study the relationship between Epstein Barr virus(EBV) and non immunodeficiency related B cell lymphoma. Methods In situ hybridization method was performed, with the probe of EBVencoded small nuclear RNAs(EBERs).Immunohistochemistry stain was used to detect immunophynotype.Results All cases were B cell origin, half of the cases were low grade (centroblastic centrocytic 12 cases,centrocytic 4 cases and lymphocytic 4 cases);20 cases were high grade (centroblastin 17 cases, immunoblastic 2 cases and Burkitt's like lymphoma 1 case).EBERs ISH showed EBV infected cells in 3 of 40 cases(1/20 cases in low grade,2/20 cases in high grade), with the proportion of the infectde cells ranging from 30% to 60%.Conclusion Non immunodeficiency related B cell lymphoma is probably associated with EBV infection ,but has no relationship with malignant degree.