肝胆管癌bcl-2/JH融合基因检测及bcl-2蛋白表达研究

Detection of bcl- 2/JHfusion gene and bcl- 2 protein expression in cholangiocellular carcinoma

探讨ｂｃｌ－ ２ 基因重排及ｂｃｌ－ ２ 蛋白表达与肝胆管癌发生的关系，采用半套式原位聚合酶链反应和免疫组织化学技术对２１ 例肝胆管癌中ｂｃｌ－ ２／ＪＨ 融合基因和ｂｃｌ－ ２ 蛋白表达进行原位观察。结果：２１ 例肝胆管癌中ｂｃｌ－ ２ 蛋白阳性１５ 例，阳性率为７１－４ ％ ，未发现ｂｃｌ－ ２／ＪＨ 融合基因。ｂｃｌ－ ２ 蛋白表达与ｂｃｌ－ ２ 基因重排之间无相互对应关系。提示肝胆管癌发生可能与ｂｃｌ－ ２ 蛋白的异常表达关系密切，而与ｂｃｌ－ ２ 基因重排无关。

To detect the relationship between bcl- 2 gene rearrangement and bcl- 2 protein ex pression and cholangiocellular carcinoma(CCC) .Methods:Im munohistochemistry and Semi- nested in situ PCRtechniques wereusedto detected bcl- 2/JHfusion gene and bcl- 2 proteinin 21 casesofCCC. Results:71-4 % (15/21)of CCCexpressed bcl - 2 protein,but no bcl- 2/JHfusion gene was found in CCC.There was no corresponding relation between bcl- 2 protein expression and bcl - 2 gene rear rangement.Conclusion:bcl - 2 overexpression was