肝癌中IGF-Ⅱ表达与11p染色体脆性位点、癌断点的关系

Astudy ofthe Relationship Between Expression of IGF- Ⅱand Fragile Sites,Cancer Breakpoints at Short Arm of Chromosome 11 in Hepatocellular Carcinoma

探讨人肝癌组织胰岛素样生长因子Ⅱ（ＩＧＦ－ Ⅱ） 的表达与１１ 号染色体短臂（１１ｐ） 结构改变的关系，用免疫组织化学ＳＰ法和核型分析法分别检测了同一肝癌（ＨＣＣ） 患者癌组织ＩＧＦ－ Ⅱ和１１ｐ 部位的脆性位点、癌断点。２５ 例（ｎ ＝ ３０）ＩＧＦ－ Ⅱ阳性ＨＣＣ中，伴脆性位点１１ｐ１３ 者３例，伴癌断点１１ｐ１５ 者１ 例，同时伴有脆性位点１１ｐ１３ 或１１ｐｌ５ 和癌断点１１ｐ１２ － １５ 者４ 例，５ 例ＩＧＦ－ Ⅱ阴性ＨＣＣ中仅１ 例有脆性位点１１ｐｌ４ 。１１ｐ 结构变化与ＩＧＦ－ Ⅱ异常表达在人肝细胞癌变中可能有重要的作用。

To investigate the relationship between insulin -like growth factor Ⅱ(IGF- Ⅱ)gene expression andfragilesites(fra),cancerbreakpointsatshortarm ofchromosome 11(11p)in human hep atocellularcarcinoma(HCC).The expression ofIGF- Ⅱin tumortissueandfragilesitesfrom peripheral bloodlymphocyteand cancer breakpointsfrom thesame HCCpatient were detected by the method of SP im munohistochemistry and karyotypicanalysisrespectively .IGF- Ⅱshowed positivestaining in the tu mortissues of25 cases (n= 30) ,whichfour had both fra(11pl3) orfra (11pl5) and cancer breakpoints at11p12 - 15 ,three with fra(11pl3) ,one with breakage at 11pl5 ;One had fragilesite(11p14)of IGF- Ⅱstaining negativein HCCs(n = 5) .Thesefindingsindicatethatthe alterationsatchromosomal11 may play animportantrolein theexpression and they mayrelatedtothe malignanttransformation of hepato cytes.