P选择素及其单克隆抗体对肝、肾缺血-再灌注时细胞凋亡的影响

Role of Pselectin and antiPselectin monoclonal antibody in apoptosis during liver and renal ischemiareperfusion injury

目的：探讨Ｐ选择素及其单克隆抗体在肝、肾缺血再灌注时其表达对细胞凋亡的影响。方法：建立大鼠肝及肾缺血再灌注损伤模型，分别采用免疫组化链菌素抗生物素蛋白生物素酶标法和原位ＤＮＡ片断末端标记法检测有或无Ｐ选择素单抗治疗组肝、肾组织中Ｐ选择素表达及凋亡细胞。结果：大鼠肝、肾缺血再灌注时Ｐ选择素在肝、肾内广泛表达，肝、肾组织出现显著病理改变并发生细胞凋亡；Ｐ选择素单抗作用组肝、肾组织中Ｐ选择素未表达，无明显病理变化且细胞凋亡减少。结论：在肝、肾缺血再灌注时，Ｐ选择素介导了肝、肾内中性粒细胞积聚和细胞凋亡，并加重肝、肾损伤；抑制Ｐ选择素可减少肝、肾内中性粒细胞积聚和细胞凋亡，减轻肝。

Objective:To evaluate the potential role of Pselectin in apoptosis during liver and renal ischemiareperfusion injury.Methods:Pselectin expression and cell apoptosis were detected in rat liver or renal ischemiareperfusion model by immunohistochemistry and terminal deoxynucleotidy transferase mediated dUTPbiotin nickend labeling(TUNEL).Results:Pselectin expression,pathological lesions and cell apoptosis were found in liver and renal tissues following liver and renal ischemiareperfusion injury,and these changes became less conspicuous in animals treated with antiPselectin monoclonal antibody.Conclusions: Pselectin might mediate neutrophil infiltration and cell apoptosis and contribute to liver and renal ischemiareperfusion injury.Inhibition of Pselectin may decrease neutrophil infiltration and cell apoptosis,thus mitigating ischemiareperfusion injury of liver and renal.