IL-3、G-CSF拮抗Ara-c诱导的白血病细胞凋亡的研究

The inhibitory effect of IL 3, G CSF on the apoptosis of leukemia cell induced by Ara c

目的：研究不同Ａｒａｃ浓度时白血病细胞凋亡的情况和ＧＣＳＦ、ＩＬ３＋ＧＣＳＦ对其的拮抗作用。方法：分别用不同剂量Ａｒａｃ诱导白血病细胞凋亡，同时加ＧＣＳＦ、ＩＬ３＋ＧＣＳＦ作为拮抗剂，分ＧＣＳＦ、ＩＬ３＋ＧＣＳＦ、Ａｒａｃ三组。通过形态学、ＤＮＡ电泳、蛋白电泳免疫印迹法观察白血病细胞的凋亡情况。结果：随着Ａｒａｃ剂量的增加，凋亡细胞数增高。ＧＣＳＦ能拮抗低剂量及常规剂量Ａｒａｃ诱导的凋亡，但不能完全拮抗中剂量Ａｒａｃ，而ＩＬ３＋ＧＣＳＦ能拮抗中剂量Ａｒａｃ。Ｂｃｌ２表达蛋白ＩＬ３＋ＧＣＳＦ组高于ＧＣＳＦ组，ＧＣＳＦ组高于Ａｒａｃ组。结论：ＩＬ３、ＧＣＳＦ能拮抗Ａｒａｃ诱导的白血病细胞凋亡。调节Ｂｃｌ２基因的表达是细胞因子拮抗凋亡的机制之一。临床应合理、适时地使用细胞因子。

Objective: To study apoptosis of leukemia cell under vary concentration of Ara c and the inhibitory effect of G CSF,IL 3+G CSF. Methods: Observed the number of apoptotic cell in leukemia induced by vary concentration of Ara c in morphology, DNA electrophoresis, immunoblotting. There were three groups: Ara c, G CSF and IL 3+G CSF. Results: The number of apoptotic cell was increased while the dose of Ara c araised. CSF can inhibit apoptosis by low and regular dose Ara c, but can not complete inhibit the middle dose Ara c. IL 3+G CSF can do it. The protein expressed by Bcl 2 in IL 3+G CSF group is higher than that in G CSF group, G CSF group is lower than Ara c group. Conclusion: This indicate that IL 3, G CSF can inhibit apoptosis of leukemia cell induced by Ara c and regulation of Bcl 2 gene expression is the one of mechanism of cytokine inhibiting apoptosis. The results suggest that cytokine be used reasonably and timely in order to gain more effective chemical treatment.