延迟性低温复合巴曲酶对沙土鼠全脑缺血后神经的影响

Effects of delayed hypothermia combined with batroxobin on behavior and histopathology following global cerebral ischemia in gerbils

目的 研究短暂全脑缺血后３０ 分开始的低温复合巴曲酶对沙土鼠行为学和组织病理学的影响。方法 采用沙土鼠全脑缺血模型，缺血时间１０ 分。动物随机分为５组：假手术组、常温组、延迟性低温组、巴曲酶组、延迟性低温复合巴曲酶组，每组７只。低温（３２℃）于再灌注３０分给予，维持３小时；巴曲酶８ＢＵ·ｋｇ－ １再灌注３０ 分经腹腔注入。于动物存活第５ 天行开阔法行为学检查，第７ 天行海马ＣＡ１区组织病理学检查。结果 行为学结果显示，常温组沙土鼠的探索活动明显较假手术组活跃；巴曲酶组或延迟性低温组沙土鼠的探索活动均较常温组明显减弱，较延迟性低温复合巴曲酶组明显活跃。组织病理学结果显示，常温组海马ＣＡ１ 区内侧、中间和外侧存活神经元计数均较常温组明显增多；延迟性低温组或巴曲酶组海马ＣＡ１ 区内侧、中间和外侧存活神经元计数均较延迟性低温复合巴曲酶组明显减少。结论 延迟性低温或巴曲酶可以减轻脑缺血后神经功能障碍和海马神经元坏死，两者复合的作用更强。

Objective The effects of delayed hypothermia combined with batroxobin on behavior and histopathology following global cerebral ischemia in gerbils were studied.Methods The ischemia was induced by occlusing the bilateral common carotoid arteries for ten minutes.Thirty five gerbils were divided into five groups:sham operated group;normothermia group;delayed hypothermia group;batroxobin group;delayed hypothermia combined with batroxobin group.Open field test were carried out at the fifth day,and histopathology test at the seventh day.Results The exploring activities of gerbils in both batroxobin and delayed hypothermia group were lower than that in normothermia reperfusion group,but higher than in hypothermia combined with batroxobin group.Animals of batroxobin group or delayed hypothermia group showed less neuron loss of CA1 than that of normothermia group,but more neuron loss of CA1 than that of hypothermia combined with batroxobin group.Conclusion Batroxobin or delayed hypothermia provides neuroprotective effects from the behavior deficits as well as neuronal injury following transient global cerebral ischemia,and their efficacy is substantially elevated when the treatments are combined.