流式细胞术检测大肠癌患者细胞免疫表型

Detection of Immunophenotrpe by FACS in Patients with Colorectal Cancer

［目的］探讨细胞免疫功能与大肠癌患者病情变化及手术前后间的关系。［方法］用流式细胞术测定４４例大肠癌患者手术前后５种细胞免疫表型，并与正常对照组进行比较。［结果］大肠癌术前ＣＤ３＋、ＣＤ４＋、ＣＤ５６＋＋１６、ＣＤ３＋／ＣＤ２５＋细胞及ＣＤ４＋／ＣＤ８＋比值明显低于对照组，ＣＤ８＋细胞显著高于正常对照组（Ｐ＜０．０１）；术后 ＣＤ３＋、ＣＤ４＋、ＣＤ５６＋＋１６细胞及ＣＤ４＋／ＣＤ８＋比值虽有回升，高于术前（Ｐ＜０．０１），但仍明显低于对照组（Ｐ＜０．０５，Ｐ＜０．０１），ＣＤ８＋、ＣＤ３＋／ＣＤ２５＋细胞术后基本恢复正常。转移组除ＣＤ８＋外，各项指标均明显低于无转移组（Ｐ＜０．０１）。［结论］检测细胞免疫功能对大肠癌患者的手术治疗、病情判断及预后评估具有重要意义。

Purpose] To investigate the relationship among cellular immunity and stage of patients with colorectal cancer and effect of operation.[Methods] Five lymphocyte phenotypes were detected by FACS in 44 patients with colorectal cancer.A comparison study with group control was carried out. [Results]Before operation the number of CD3+, CD4+,CD56+16,, CD3+/CD25+ cells and CD4+/CD8+ rario in parients with colorectal cancer were significantly lower than those in the coned group,while the number of CD8+ cells was higher(P<0.01).After operation, though the number of CD3+, CD4+, CD56+16 cells and CD4+/CD8+ ratio increased lightly and higher than those before operation ,tray were still lower than those in the controlgroup(P<0.05,P<0.01).CD8+ and CD3+/CD25+ cells retumed to normal level,which had no signifiant difference with the control group.All types of cell except CD8+ in the patients with metastasis significanly lower than those in patients with non - metastsis (P<0.01 ).[Conclusion]Testing cellular immunity play an important role in predicting effect of opration, clinical julerat and prognostic evaluation in patients with colorectal cancer.