瓜蒌皮提取物对人脐带静脉内皮细胞损伤的保护作用

Protective effect of EPT against damages of the human umbilicus vein endothelial cellsProtective effect of EPT against damages of the human umbilicus vein endothelial cells

目的研究瓜蒌皮提取物（EPT）对氧化低密度脂蛋白（OX-LDL）诱导的人脐带静脉内皮细胞（HUVECs）损伤的保护作用。方法在培养的人脐带静脉内皮细胞中加入OX-LDL（100mg／L）诱导内皮细胞损伤。检测细胞培养液中非对称性二甲基精氨酸（ADMA）、丙二醛（MDA）、乳酸脱氢酶（LDH）、一氧化氮（NO）、二甲基精氨酸二甲胺水解酶（DDAH）和肿瘤坏死因子-α（TNF-α）的含量。结果体外给予OX-LDL（100mg／L）显著诱导内皮细胞损伤，提高培养液中ADMA、MDA、LDH、和TNF-α水平，降低NO和DDAH水平。两种洗脱（水和70％乙醇洗脱）瓜蒌皮提取物均能显著抑制OX—LDL所致的ADMA、MDA、LDH和TNF-α浓度升高和NO、DDAH降低。结论瓜蒌皮提取物对OX—LDL诱导的人脐带静脉血管内皮细胞损伤有保护作用，其保护作用与降低ADMA和TNF-α浓度有关。

Objective To study the protective effect of EPT on damages of the human umbilicus vein endothelial cells （HUVECs） induced by oxidized low-density lipoprotein （ox-LI）L）. Methods The injury of endothelial cells was induced by ox-LDL （100mg/L） in cultured endothelial cells. The medium samples were collected from cultured endothelial cells. And the concentrations of asymmetric dimethylarginine （ADMA）, malondi- aldehyde （MDA）, lactate dehydrogenase （LDH）, nitric oxide （NO）, dimethylarginine dimethylaminohydrolase （DDAH） and turnout necrosis factor-α （TNF-α） were measured. Results Ox-LDL （100 mg/L） significantly increased the concentrations of ADMA, MDA, LDH and TNF-α, and decreased the concentrations of NO and DDAH. EPT with the different elution （water and 70% alcohol） markedly reduced the injury of endothelial cells by ox-LDL, and inhibited the increase of ADMA, MDA, LDH and TNF-α, and inhibited the decrease of NO and DDAH by ox-LDL. Conclusion EPT protects the endothelial cells against damages induced by ox-LDL, and the effects is related to the reduction of ADMA and TNF-α concentration in cultured endothelial cells.