摘要
目的:评价18kDa抗原在泡型包虫病(AE)诊断中的价值。方法:用免疫印渍法检测33例泡型包虫病、69例囊型包虫病(CE)、30例囊虫病和82例健康人血清对泡型棘球蚴(Em)和囊型棘球蚴(Eg)原头节18kDa抗原的抗体反应。同时用羊包囊液抗原常规ELISA法检测上述血清。结果:ELISA试验,AE血清阳性率为93.9%、CE为85.5%、囊虫病为50%、健康人为6.1%。显示3种患者血清存在较强的交叉反应。免疫印渍试验,两种原头节的18kDa抗原对AE血清阳性反应均为90.9%、CE分别为10.1%和13%、囊虫病患者为13.3%和16.7%。与健康人血清不发生反应。结论:18kDa抗原可以有效的鉴别两型包虫病。
AIM: To evaluate the 18 kDa antigen in diagnosis of human alveolar echinococcosis. METHODS: A total of 214 sera from patients with alveolar echinococcosis (AE, 33), cystic echinococcosis (CE, 69), cysticercosis (30) and healthy controls (82) were examined by Western blotting to detect the antibodies against 18 kDa antigen using both antigens from protoscoleces of E.granulosus (Eg) and E.multilocularis (Em). All the sera were tested simultaneously by ELISA to detect the antibodies against hydatid cystic fluid antigens of sheep as a comparison. RESULTS: The positive rate by ELISA was 93 9% in AE, 85 5% in CE, 50% in cysticercosis and 6 1% in healthy controls, respectively, suggesting a critical cross reaction and a certain degree of nonspecific reaction. The serum positive rates by Western blotting with the 18 kDa antigen of Eg and Em were 90 9% and 90 9% in AE, 10 1% and 13% in CE, 13 3% and 16 7% in cysticercosis, respectively. No positive was found in the sera from healthy controls. CONCLUSION: The 18 kDa antigen may be used in the differential diagnosis of AE from CE.
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
1999年第2期78-80,共3页
Chinese Journal of Parasitology and Parasitic Diseases
基金
国家"九五"攻关资助
关键词
包虫病
AE
CE
18kDa抗原
鉴别诊断
免疫印渍
Alveolar echinococcosis, cystic echinococcosis, 18 kDa antigen, differential diagnosis, Western blotting