20种中药糖含量与寒热药性关系的Fisher判别分析

Study on Relationship Between Sugar Content and Cold-Heat Nature of 20 Kinds of Herbs by Fisher Analysis

目的:研究20种中药中糖的含量与中药寒热药性的相关性。方法:运用费林氏容量法测定20味中药总糖的含量,硫酸-蒽酮比色法测定总多糖的含量,碱式硫酸铜法测定单糖的含量,并利用Fisher统计方法分析实验数据。结果:10种热性中药的总糖和单糖含量都明显高于10种寒性中药,统计分析显示:P<0.05;总多糖含量测定的统计结果显示:P>0.05;Fisher法建立判别函数,判别正确率为85%。结论:Fisher统计分析方法可以从糖含量方面对20味中药的寒热药性进行判别,20种中药中糖的含量与寒热药性具有相关性,提示糖类物质是寒热药性的物质基础之一。

Twenty kinds of herbs were studied for the relationship between sugar content and Cold-Heat nature. Th.e Fehling method was used for determining the total sugar content, the sulfuric acid-anthraquinone colorimetric method for measuring the total polysaccharides content, and the alkaline copper sulfate method for testing the monosaccharides content. After that, the obtained data were analyzed by the Fisher method. The 10 kinds of Heat herbs showed significantly higher total sugar content and the monosaccharides content than the 10 kinds of Cold herbs, with P〈0.05; however, they showed no significant differences in the total polysaccharides content, with P〉 0.05. A discriminant function was established by the Fisher method, with the group back substitution result of the Fisher analysis reaching 85%. The results demonstrate that there exists a certain relationship between the sugar content and the Cold-Heat nature of the 20 kinds of herbs, the Fisher analysis can be used to distinguish the Cold- Heat nature, and sugar is one of the material bases of Cold-Heat nature.