我国流行性喘憋性肺炎呼吸道合胞病毒分离株F蛋白基因特征初探

A preliminary study on the characteristics of fusion protein genes of respiratory syncytial virus field strains isolated from children with epidemic asthmalike pneumonia

目的探讨流行性喘憋性肺炎（简称流喘肺炎）与散发肺炎呼吸道合胞病毒（ＲＳＶ）分离株Ｆ蛋白的异同。方法采用ＰＣＲ片段直接测序及克隆测序，对１１株流喘肺炎ＲＳＶ分离株及５株散发肺炎ＲＳＶ分离株Ｆ蛋白基因序列进行了分析比较。结果被检测流喘肺炎ＲＳＶ分离株中，３６％（４／１１）的毒株其Ｆ蛋白基因３′非编码区（３′ＮＣＲ）（第１７４１位核苷酸）存在６个核苷酸插入，而散发肺炎ＲＳＶ分离株均无此插入存在。结论ＲＳＶ流喘肺炎分离株与散发肺炎分离株Ｆ蛋白基因的３′ＮＣＲ有差异。

Objective To identify genetic differences among the fusion (F) protein genes of respiratory syncytial virus (RSV) field strains isolated from pediatric patients with epidemic asthmalike pneumonia and from regular pneumonia. Methods Direct sequencing of PCR products from partially amplified F genes and sequencing of cloned cDNA from F genes were used in this study. Results Among 11 strains isolated from epidemic asthmalike pneumonia, 4 (36%) had extra six nucleotides inserted into the 3end noncoding region of the F gene, whereas none of the F genes from 5 strains isolated from regular pneumonia had that insertion. The 2 test showed P>0.05. Conclusion There were differences among the F genes of RSV isolates from epidemic asthmalike pneumonia compared with those from regular pneumonia.