市售壁虎不同剂型对S180荷瘤小鼠作用的实验研究

Experimental study on anti-tumor effect of different preparations of gecko from market on sarcoma S180 mice

目的:探讨市售中药壁虎的三种剂型散剂、水煎液和酒剂对S180荷瘤小鼠的作用。方法:建立移植瘤小鼠S180肉瘤模型和S180腹水瘤模型。将90只ICR小鼠和90只昆明种(KM)小鼠随机分为模型组,酒对照组,环磷酰胺(CTX)组,壁虎水煎液、散剂和酒剂高、低剂量组,分别以生理盐水、56度白酒及不同剂型的壁虎灌服荷瘤小鼠10d(CTX组20mg/kg,连续5次),测定抑瘤率、胸腺指数、脾指数和瘤组织血管内皮生长因子(VEGF)含量,对腹水瘤模型KM小鼠进行生存期分析。结果:与模型组比较,本实验所使用的市售中药壁虎各剂型对S180移植瘤模型的瘤重、瘤组织VEGF含量及生存期无明显影响,9组小鼠的胸腺及脾指数没有明显差别。结论:目前市售中药材壁虎的体内抑瘤活性尚不确定,影响壁虎抗肿瘤活性的相关因素有待进一步的研究。

Objective：To investigate the anti-tumor activity of three preparations of gecko from market on sarcoma S180 mice.Methods：Mice xenotransplant model of sarcoma S180 and mice ascites tumor of sarcoma S180 were established.Ninety mice were randomly divided into 9 groups：control group,wine control group and CTX group,the other six groups were orally administrated by three preparations of gecko,each at two doses（low and high）.Rate of restraining tumor,index of thymus and spleen,rate of survival prolonged and VEGF content in sarcoma were calculated after 10 days’treatment.Results：Processed gecko from market had no significant impact on rate of restraining tumor and VEGF content in sarcoma,it also can not prolong survival time.There was no significant difference among the nine groups in the index of thymus and spleen.Conclusion：Whether processed gecko from market had anti-tumor activity in vivo remained further explored.