养心草药材HPLC指纹图谱研究

Studies on HPLC fingerprint of Herba Sedum aizoon L

目的:采用HPLC法对不同采收期的养心草药材进行指纹图谱研究,为养心草药材质量控制提供依据。方法:采用DiamonsilC18色谱柱(4.6mm×250mm,5μm),以乙腈-0.5%磷酸水为流动相进行梯度洗脱,流速1.0mL∕min,柱温25℃,检测波长254nm。结果:以21个共有峰为评价指标,精密度和重复性实验中各共有峰相对保留时间和相对峰面积的RSD均小于3%,10批样品相似度大于0.9。结论:此方法精密度、稳定性和重复性良好,有助于养心草药材的质量控制。

Objective：The fingerprint chromatograms were established for quality evaluation of Sedum aizoon L.collected from different habitats by HPLC.Methods：The analysis was performed on a Diamonsil C18 column（4.6mm× 250mm,5μm）with acetonitrile-water（acidified to 0.5%with phosphoric acid）as mobile phase in a gradient mode at a flow rate of 1.0 mL/min,and at a column temperature of 25℃.The detection of wavelength was at 254 nm.Results：2lpeaks were selected as the common fingerprint peaks.The relative standard deviations for relative retention values and relative peak areas were less than 3%in the precision and repeated test.The similarity of l0 batches of samples were no less than 0.9.Conclusion：The method was reliable and can be helpful on the quality control of Sedum aizoon L.