摘要
目的:研究镉导致核苷酸切除修复基因XPC在HEK293细胞中的表达变化,探讨XPC基因在镉导致细胞DNA突变种的作用。方法:离体培养的人胚肾293细胞以20umol/L(低剂量组)和40umol/L(高剂量组)分别氯化镉处理24小时,Real-timePCR检测处理前后XPC的表达变化情况。结果:两组中,氯化镉处理前后XPC的表达明显升高(P<0.01);高剂量组中XPC的表达与低剂量组中XPC的表达没有明显差别(P>0.05)。结论:高表达的XPC、XPF在镉导致的DNA突变中起着重要的修复作用,核苷酸切除修复机制对镉造成的DNA损伤具有一定的修复作用。
Objective： Little is known now about the function of NER in the HEK293 cell induced by the cadmium, we investigated the mRNA expression of Xeroderma Pigmenosum group C in HEK293 cell induced by cadmium in different concelttration. Method： Transformed human embryonic kidney 293 cell line were incubated with 40μ mol/L or 80μ mol/L CdCl 2 for 24 hours Expression levels of xpc were detected with the real-time polymerase chain reaction. Results： The xpc gene was frequently overexpressed in HEK293 cell induced by cadmium compared to normal HKE293 cell. Conclusion： Our findings suggest that overexpression of xpc gene involved in the early steps of NER process and could be related to the well recognized resistance of cadmium in some degree.
出处
《现代生物医学进展》
CAS
2010年第16期3015-3016,共2页
Progress in Modern Biomedicine
基金
国家自然科学基金项目(30772160)
