摘要
目的:探讨丙戊酸钠协同阿霉素对MUTZ-1细胞株生长的抑制作用及其作用机制。方法:采用四甲基偶氮唑蓝(MTT)比色法观察药物对细胞生长的影响;发光比色法检测Caspase-3的活性。结果:小剂量丙戊酸钠可协同阿霉素抑制MUTZ-1细胞株生长,细胞生长抑制率在两药联合作用后比单独使用阿霉素明显增加,有剂量依赖性,与对照组相比有显著统计学意义。单药使用阿霉素时细胞的caspase-3活性上升,两药联合后caspase-3活性上升更明显。结论:小剂量的丙戊酸钠能增敏阿霉素诱导MUTZ-1细胞株发生凋亡,作用机制可能是通过上调caspase-3的活性来完成的。
Objective： To investigate the tumor suppression efficacy of histone deacetylase inhibitor,valproate sodium,in combination with Adriamycin in the treatment of myelodysplastic syndrome cell line MUTZ-1 and its mechanism. Methods： Logarithmic growth phase MUTZ-1 cell line was treated on different density of valproic acid,Adriamycin,alone or combination;after alone and combination. Caspase-3 activity was detected. Results： 0.25mmool/l valproate sodium did not show apoptosis effect, but can synergize Adriamycin to promote Apoptosis. it was concentration -dependent. All the data above were significantly different from that in control （ p〈 0.05）. Aider treated with SVPA and ADM for 72h, the level ofcaspase-3 in MUTZ-1 was enhanced （P〈0.05）. Conclusions： valproate sodium may enhance the efficacy of ADM in MUTZ-1 cell line. the mechanism of action is possibly through the activity ofcaspase-3.
出处
《现代生物医学进展》
CAS
2010年第16期3033-3036,共4页
Progress in Modern Biomedicine
基金
国家自然基金资助项目(30070318)
