Polyporus sp．M05多糖的抗肿瘤作用

Anticancer effect of polysaccharide isolated from Polyporus sp. M05

目的研究真菌Polyporus sp．M05多糖PSM—a体内对荷瘤小鼠S180的抑瘤作用及其机制。方法MTT法检测PSM-a体外对S180细胞株的增殖抑制作用。建立S180小鼠模型，灌胃治疗，每日检测肿瘤体积并计算瘤体比和抑瘤率。实验结束后处死小鼠，MTr比色法测定小鼠自然杀伤（NK）细胞及淋巴因子活化杀伤细胞（LAK）的杀伤活性。HE染色检测肿瘤组织细胞坏死情况。结果PSM-a在体外能抑制S180细胞生长；在体内能显著降低荷瘤小鼠的瘤重和瘤体比，250μg／ml的PSM-a对肿瘤抑制率高达80％以上；PSM-a能促进NK细胞及LAK细胞的杀伤活性；病理切片显示PSM-a作用后引起肿瘤组织坏死。结论PSM-a对S180荷瘤小鼠肿瘤的生长有明显的抑制作用，其机制可能与提高机体免疫细胞对肿瘤细胞的杀伤活性有关。

Objective To study effect and mechanism of fungus polysaccharide PSM-a of Polyporus sp. M05 on S180 bearing mice. Methods MTT method was used to detect the inhibiting role of PSM-a on S180 cells proliferation in vitro. S180 mice model was established, and was administered by gavage. Tumor volume was detected, and the ratio of tumor to mile weight and inhibiting tumor rate. The activity of NK and LAK cells on the target cells was analyzed by MTT colorimetric assay; HE stain was used to detect the necrosis of tumor cells. Restilts PSM-a could inhibit S180 cells grouth in vitro. PSM-a could decrease the tumor weight and increase the ratio of tumor volume and mice weight; Tumor inhibiting rate reached 80% and above when treated with 250μg/ml PSM-a. PSM-a could increase the activity of NK and LAK cells, and necrosis happened. Conclusion PSM-a could significantly inhibit the growth of S180 cells, and the mechanism bnay be related with the increased killing activity of immunne cells to tumor cells.