聚集素和Caspase-3在非小细胞肺癌中的表达及意义

The expression and significance of Clusterin and Caspase-3 in non-small cell lung cancer

目的探讨聚集素(C lusterin)和Caspase-3蛋白在非小细胞肺癌(NSCLC)组织中的表达及与临床病理特征之间的关系及意义。方法采用免疫组织化学方法(SP法)检测C lusterin与Caspase-3蛋白在52例NSCLC组织和20例正常肺组织中的表达情况,结合临床病理资料进行分析。结果 52例肺癌组织中C lusterin阳性表达率为78.85%,20例正常肺组织中的阳性表达率为10%,两者有显著性差异(P<0.05),C lusterin的阳性表达与肿瘤的分化程度、TNM分期及淋巴结转移有关(P<0.05),而与肿瘤的病理类型无关(P>0.05)。52例NSCLC组织中Caspase-3的阳性表达率为44.23%,20例正常肺组织中的阳性表达率为80%,两者差异显著(P<0.05),Caspase-3的阳性表达与肿瘤的分化程度、淋巴结转移有关(P<0.05),而与肿瘤的病理类型、TNM分期无关(P>0.05)。C lusterin与Caspase-3在NSCLC组织中的表达呈负相关。结论 C lusterin过表达及Caspase-3的失表达可能促进了肿瘤的恶性增殖及转移,联合检测C lusterin与Caspase-3对判断NSCLC恶性程度、转移潜能及预后有重要临床意义。

Objective To investigate the correlatetion of the expression and clinicopathologic significance of clusterin and caspase-3 protein in non-small cell lung cancer（NSCLC）.Methods The expression of clusterin and caspase-3 protein in 52 cases of lung cancer and 20 cases of normal lung tissue were detected by SP immunohistochemistry method,and the clinicopathologic features of lung cancer were analyzed statistically.Results The positive rate of clusterin protein expression in 52 cases of NSCLC was 78.85%,while in benign tissue was 10%（P〈0.05）;the positive rate of clusterin protein expression was closely related to degree of cell differentiation,TNM staging,and lymph node metastasis（P〈0.05）,while not to tumor pathological types（P〉0.05）.The positive rate of caspase-3 protein expression in 52 cases of NSCLC was 44.23%,while in benign tissue was 80%（P〈0.05）;the positive rate of caspase-3 protein expression was closely related to degree of cell differentiation,and lymph node metastasis（P〈0.05）,while not to tumor pathological typers and TNM staging（P〉0.05）.Negative correlations were observed between the expression of clustein and caspase-3 in NSCLC（P〈0.05）.Conclusion Overexpression of clusterin and low expression of caspase-3 may promote the malignant hyperplasy and metastasis of tumor.Co-detected method of the expression levels of the two proteins in NSCLC has important significance in analyzing the degree of malignancy,the potential capacity of metastasis and prognosis.