玉屏风口服液对口腔扁平苔藓差异表达基因干预的初步探讨

Effects of Yupingfeng oral liquid on differentially expressed genes in oral lichen planus

目的应用基因芯片技术及最新公共数据库,观察玉屏风口服液作用后口腔扁平苔藓(oral lichen planus,OLP)组织中基因表达谱改变。方法分离纯化正常口腔黏膜组织、口腔扁平苔藓病变组织和服用玉屏风口服液后扁平苔藓病变组织mRNA,制备表达谱探针,混合后用BiostarH-40s型基因芯片杂交,用ScanArray 4000荧光扫描仪扫描芯片荧光信号图像,利用GenePix Pro 3.0软件分析检测玉屏风口服液作用口腔扁平苔藓组织中的差异表达基因,并进行筛选。结果服用玉屏风口服液后,病变组织有5条基因表达上调,3条基因表达下调,功能以转录基因为主。结论玉屏风口服液对口腔扁平苔藓的差异表达基因具有干预作用,干预基因以转录基因为主。

Obiective To observe the changes of gene expression profile of oral lichen planus after Yupingfeng oral liquid treatment by cDNA microarray.Methods The total RNAs were isolated from normal oral mucosa tissue,oral lichen planus tissue and oral lichen planus tissue after Yupingfeng oral liquid treatment.Then they were reversely transcribed to prepare the hybridization probe.The mixed probes were hybridized with the BiostarH-40s cDNA microarray.After washing,the cDNA microarray was scanned for the fluorescent signals by ScanArray 4000 scanning apparatus.Genepxix Pro 3.0 software was used to detect and select the genes differentially expressed in oral lichen planus after Yupingfeng oral liquid treatment.Results The result indicated that the expressions of 5 genes were up-regulated and expressions of 3 genes were down-regulated after Yupingfeng oral liquid treatment.Conclusions Yupingfeng oral liquid plays an impotant role in intervening the differentially expressed genes in OLP and the genes intervened are mainly transcription factors.