明胶壳聚糖可吸收膜支架缓解兔眼实验性滤过泡瘢痕化的作用

Alleviate the rabbits eyes of filtering bleb scarring by Chitosan gelatin absorbable stent

目的:解明胶壳聚糖可吸收膜支架对缓解兔眼实验性滤过泡瘢痕化的作用。方法:制备明胶壳聚糖可吸收膜,兔眼滤过手术中植入结膜下,并设立对照组。术后裂隙灯观察滤过泡情况。术后7,14d摘除眼球后常规病理HE染色,计算滤过泡腔面在结膜中形成的面积百分比,并按照独立样本t检验分析明胶壳聚糖可吸收膜对滤过泡形成及纤维化的影响。结果:术后7d实验组及对照组均可见弥散隆起的滤过泡。术后14d实验组可见滤过泡扁平,对照组滤过泡完全消失。术后7d实验组与对照组在高倍视野下滤过泡腔面积在结膜中形成的面积百分比分别为6.16%±1.02%,5.96%±0.85%,统计学分析无明显差异。术后14d实验组滤过泡腔面积在结膜中形成的面积百分比1.09%±0.13%,对照组可见大量纤维组织增生,未见滤过泡,根据独立样本t检验,t=8.52,P=0.012,实验组的滤过泡面积百分比明显高于对照组。结论:明胶壳聚糖可吸收膜的支架作用对术后滤过泡的维持及防止滤过泡瘢痕化有效。

AIM： To understand the effect of Chitosan gelatin absorbable stent on alleviating the rabbits eyes of filtering bleb scarring. METHODS： Preparation of Chitosan gelatin absorbable membrane as implants were applied in rabbit eye filtration surgery under the conjunctiva ,and the control group was established. The postoperative filtering bleb condition was observed by slit-lamp. Eyes were removed to stain with HE on postoperative day 7 and 14, and the percentage of the filtering bleb area in the conjunctiva was calculated,and the effect of Chitosan gelatin absorbable membrane was analyzed according to the filtering bleb formation and fibrosis by independent-samples t test . RESULTS： In the postoperative day 7, the bulging diffuse bleb could be seen in both groups. In the postoperative day 14, flat bleb could be seen in the experimental group and no bleb but a large number of fibrous tissue in the control group. The percentage of the filtering bleb area in the conjunctiva were 6.16%±1.02%, 5.96%±0.85% in the experimental and control group, respectively in the postoperative day 7 and there was no statistical difference.However, in the postoperative day 14, The percentage of the filtering bleb area in the conjunctiva was 1.09%±0.13% in the experimental group, but no filtering bleb in the control. The percentage was higher in the experimental group than in the control group by the independent-samples t test （t=8.52 and P=0.012）. CONCLUSION： It is effective to prevent filtering bleb scarring with the Chitosan gelatin absorbable membrane.