胚胎多巴胺神经元移植治疗帕金森病的实验研究

Intracerebral transplantation of embryonic dopamine neurons for Parkinson disease:An experimental study

目的探讨胚胎多巴胺神经元移植对帕金森病大鼠的治疗作用。方法立体定向注射6-羟多巴胺建立帕金森病大鼠模型,随机分为对照组(n=12)和细胞移植组(n=12)。细胞移植组将荧光染料CM-DiI标记的大鼠胚胎多巴胺神经元立体定向注入帕金森病大鼠纹状体区,对照组于相同部位注入生理盐水。用阿扑吗啡诱导帕金森病大鼠旋转行为评估细胞移植的治疗作用。细胞移植8周后取大脑标本行冷冻切片,荧光显微镜下观察移植细胞在脑内存活情况。结果与对照组比较,移植多巴胺神经元能显著改善阿扑吗啡诱导帕金森病大鼠的异常旋转行为(P<0.01)。移植8周后,仅少量多巴胺神经元存活。结论多巴胺神经元移植可短期内改善帕金森病大鼠的运动障碍,但长期疗效不佳,可能与移植多巴胺神经元长期存活率较低有关。

Objective To explore the therapeutic effect of transplanted dopamine（DA）neurons in Parkinson disease（PD）rat models.Methods The PD rat models were established by stereotactic injection of 6-hydroxydopamine and then were randomly divided into control group（n=12）and cell transplanted group（n=12）.The rat DA neurons labeled by fluorescent dye CM-DiI were injected stereotactically into the corpus striatum of PD rats in cell-transplanted group and physiologic saline was injected into the same site of rats in control group.The therapeutic effect of cell transplantation was evaluated through rotations of PD rats induced by apomorphine（APO）.Frozen sections of the brain samples were prepared 8 weeks after transplantation and the survival of transplanted cells was observed under fluorescence microscope.Results Compared with control group,the transplanted DA neurons more significantly improved the abnormal rotational behavior of PD rats induced by APO（P〈0.01）.Eight weeks after transplantation,only a few transplanted cells survived.Conclusions Transplantation of DA neurons can significantly improve abnormal behavior of PD rats in short term.However,the long-term therapeutic effect is poor,which may be related to the low survival rate of transplanted DA neurons in long term.