LPA2受体介导LPA诱导的人支气管肺上皮细胞PAI-1表达

Effect of LPA2 on LPA-induced PAI-1 mRNA Expression in Human Bronchial Epithelial Cells

目的研究溶血磷脂酸(LPA)对原代培养人支气管肺上皮细胞(NHBE)表达纤溶酶原激活物抑制因子-1(PAI-1)的调节作用。方法原代培养人支气管上皮细胞培养于6孔板培养板内,经LPA诱导后,收取RNA裂解液并用实时荧光定量RT-PCR技术检测PAI-1 mRNA表达水平;利用RNA干扰技术研究LPA受体的作用。结果 LPA可诱导NHBE细胞PAI-1 mRNA表达上调且呈时间相关性,此效应可被LPA2 siRNA完全阻断。结论 LPA2受体介导LPA诱导NHBE细胞表达PAI-1。

Objective To investigate the effect of lysophosphatidic acid（LPA） and the role of LPA receptor 2（LPA2） on plasminogen activator inhibitor-1（PAI-1） expression in human bronchial epithelial cells（NHBE）.Methods NHBE cells were cultured in 6-well culture plate and stimulated with LPA.After stimulation,cells were collected for PAI-1 mRNA detection by quantitative real time RT-PCR.The role of LPA receptor was further investigated by using RNA interference technique.Results LPA can induce NHBE PAI-1 mRNA expression in a time-dependent manner,and this effect can be inhibited by LPA2 siRNA.Conclusion LPA2 mediates LPA-induced NHBE PAI-1 mRNA expression.