苦参与茯苓对肝纤维化的作用及机制的研究

Effects of Sophora Flavescens and Tuckahoe on Hepatic Fibrosis

目的研究苦参、茯苓两种中药抗肝纤维化的作用及其机制。方法以生理盐水作为对照,将苦参和茯苓两种中药用于大鼠肝纤维化模型和大鼠肝星状细胞(HSC)。观察肝组织形态变化;检测血透明质酸酶、Ⅳ型胶原含量;MTT法检测HSC细胞增殖抑制率;免疫组化法检测基质金属蛋白酶-1(MMP-1)、基质金属蛋白酶组织抑制因子-1(TIMP-1)、转化生长因子(TGF)β1、血小板衍生生长因子(PDGF)的表达。结果与对照组比较,中药处理组的HSC细胞增殖抑制率升高(P<0.05);血清透明质酸、Ⅳ型胶原含量减少(P<0.05);肝组织TIMP-1、TGFβ1及PDGF的表达均减少(P<0.05)。结论两种中药均能减缓大鼠肝纤维化的发生,其机制可能与这些中药能下调TGFβ1及PDGF表达、抑制HSC增殖活化、促进细胞外基质降解、减少肝纤维结缔组织沉积有关。

Objective To study the mechanisms and anti-fibrotic effect of Sophora flavescens and Tuckahoe.Methods The 2 Chinese traditional drugs were given to rats with hepatic fibrosis.Normal saline was administered to the control rats.Degree of hepatic fibrosis,serum levels of hyaluronic acid（HA） and type IV collagen;expression of tissue inhibitors of matrix metalloproteinase-1（TIMP-1）,matrix metalloproteinase-1（MMP-1）,transforming growth factor β1（TGFβ1） and platelet-derived growth factor（PDGF） in liver tissues were measured.HSC growth rate were（measured） by MTT method.Results Compared with the control group,the serum level of HA and type IV collagen were significantly decreased.Expression of TIMP-1,TGFβ1 and PDGF were decreased significantly in all the treatment groups（P0.05）.Conclusion The drugs could inhibit hepatic fibrosis.The inhibitory effect may be due to the reduction in the expression of TGFβ1,PDGF,and also the ratio of TIMP-1 to MMP-1.