间接免疫荧光检测技术检测沙门菌的实验研究

Application of Indirect Immunofluorescent Assay for the Detection of Salmonella

目的为加强食品安全管理,快速检测食源性致病菌,对间接免疫荧光检测法检测沙门菌的敏感性进行探讨。方法制备沙门菌抗原片和食品样品抗原片,用沙门菌A-F多价诊断血清作为一抗,异硫氰酸荧光素(FITC)标记羊抗兔IgG作为二抗。结果与国标法比较,沙门菌间接免疫荧光法具有快速、简便等优点,且两种检验方法的阳性检出率未出现统计学差异(P=0.250)。结论沙门菌间接免疫荧光检测法可以用于食品安全快速检测。

Objective Develop indirect immunofluorescent technique for the rapid detection of Salmonella and to strengthen the management of food safety.Methods Salmonella antigens and antigens from food samples were prepared and coated on slides.Salmonella A-F diagnostic antisera was used as primary antibody and fluorescein isothiocyanate（FITC） conjugated sheep IgG was used as secondary antibody in the immunofluorescent assay.Results There was no statistics significance between the developed indirect immunofluorescent assay and the National Standard Method（P= 0.250）.Compared with the National Standards Method,the indirect immunofluorescent assay were rapid and simple to perform.Conclusion The developed indirect immunofluorescent method may be used for the rapid detection of foodborne salmonella safety.