戊型肝炎多重诊断模式的研究

Research of Multiple Diagnostic Methods for the Diagnosis of Hepatitis E

目的探索一种基于ELISA和核酸定性检测技术的戊型肝炎多重诊断模式。方法对261份血清标本采用ELISA技术检测血清戊型肝炎病毒IgM抗体,采用巢式PCR技术对血清中戊型肝炎病毒核酸定性检测。结果 2份血清标本IgM抗体检测呈弱阳性和核酸检测均呈阳性,阳性率为1.0%。结论戊型肝炎病毒IgM抗体和核酸检测相结合的方法有助于戊型肝炎早期诊断和传染风险性评估。

Objective To develop the detective method of multiple hepatitis E virus by using ELISA and RT-PCR.Methods The level of IgM antibody from 261 blood serum specimens was evaluated using the ELISA method.Hepatitis E viral genome was detected using the nested PCR.Result Two blood serum specimens exhibited a weak positive IgM reaction in the ELISA assay and the positive rate was 1.0%.Conclusion Both ELISA and RT-PCR are useful in the early diagnosis of hepatitis E.