强冷自身抗体合并同种抗体引起配血困难一例分析

Analysis One Case of Strong Cold Autoantibodies Combined with Alloantibody Cause Transfusion Difficulty

目的通过对患者自身抗体和同种抗体的血清学检测特性的分析,使临床能够及时、有效的安全输血。方法将患者血清标本在室温、4℃、37℃用盐水、抗人球、聚凝胺等方法进行抗体鉴定以及交叉配血试验。结果强冷自身抗体在室温、4℃凝集所有红细胞,经过温浴后,可以检测到被掩盖的同种抗体的存在。结论对有多次输血史或妊娠史的病人应予以高度重视,要用聚凝胺法、抗人球法或微柱凝胶法等做交叉配血实验,应排除自身抗体后,再进行抗体鉴定,确保输血安全。

Objective To insure the clinical blood transfusion safety and effectiveness by analyzing the serological test characteristics of the patient＇s autoantibody and alloantibody.Method The antibody identification test and the cross matching test were done with saline water method,coombs test,and polybrene method at room temperature,4℃ and 37℃.Result Strong cold antibody could agglutinate the red blood cells at room temperature and 4℃,and the alloantibody which was masked could be detected after a warm bath.Conclusions We must pay highly attention to those patients with the history of blood transfusion and pregnancy.We should use polybrene method,coombs test and microcolumn gel to do the cross matching test.When doing the test,we should first exclude the autoantibody,and floow by the antibody identification test to insure the safety of the blood transfusion.