阿托伐他汀对脓毒症大鼠血浆一氧化氮的影响

Influence of atorvastatin to the nitric oxide in blood plasma of sepsis rats

[目的]探讨阿托伐他汀对脓毒症大鼠血浆一氧化氮(NO)的影响。[方法]健康雄性大鼠75只,随机分为对照组、脓毒症组、阿托伐他汀组,每组25只。对照组行假手术;脓毒症组行盲肠结扎穿孔术(CPL);阿托伐他汀组,用阿托伐他汀灌胃,行盲肠结扎穿孔术(CPL)。分别在术后0、2、6、10、24 h时间点采血,检测血浆NO水平。取肺、空肠标本,HE染色后,光镜下检查组织病理学改变。[结果]NO浓度在脓毒症组血浆术后6 h明显升高,24 h达到峰值(329.04±21.14)μmoL/L;在阿托伐他汀组术后10 h明显升高,在24 h达到峰值(209.53±10.68)μmoL/L。在6 h,10 h,24 h时间点,两组比较,差异有显著性意义(P<0.01)。病理改变:对照组无明显病理改变,脓毒症组肺、肠壁组织坏死充血,炎细胞浸润,阿托伐他汀组炎症反应介于两者之间。[结论]阿托伐他汀可能延缓脓毒症大鼠血浆NO上升速度,减轻炎症反应。

[Objective] To investigate the influence of atorvastatin on nitric oxide（NO） in blood plasma of sepsis rats.[Methods] A total of 75 male rats were randomized in control group,sepsis group and atorvastatin group（n=25）.The rats were treated with sham-operation in control group,with（CPL） in sepsis group,and feeded on 40 mg kg-1 atorvastatin with intragastric administration before CPL in atorvastatin group.The blood of the rats were colleced in postoperative 0 h,2 h,6 h,10 h,24 h in the groups.The levels of NO were detected with the spectrophotometera.The specimen of the lung and jejunum of the rats were pathological examination.[Results] The level of NO significantly increases in sepsis group at postoperative 6h and get peak（329.04±21.14）μmol/L at 24 h.The level of NO increases in atorvastatin group at postoperative 10h and get peak（209.53±10.68）μmol/L at 24 h.Compared with the sepsis group,the levels of NO in atorvastatin group were significantly lower（P0.01） at 6 h,10 h and 24 h.Hyperemia,necrosis and exudate on the specimen of the lung and jejunum were shown in sepsis group,but were reduced in atorvastatin group.[Conclusions] Atorvastatin may postpone of the NO in blood plasma in sepsis rats and reduce inflammatory reaction.