珠美海棠MzWRKY基因家族盐胁迫应答模式研究

Expression Analysis of WRKY Family in Response to Salt Stress in Malus zumi Mats

对苹果属植物现有的58个WRKY的EST序列进行分析,得到了37条uniESTs。珠美海棠(Malus zumi Mats)是耐盐的优良苹果砧木,能在土壤全盐含量为0.6%的盐碱地上存活。根据已知的37条uniESTs序列,通过半定量RT-PCR研究了珠美海棠中MzWRKY基因家族的盐应答模式。28个MzWRKY基因能检测到RT-PCR的产物,其中21个基因受盐诱导,1个受盐抑制。根据MzWRKY基因盐应答模式可将这些基因分为两组,基因表达模式的差异说明MzWRKY在珠美海棠的盐应答中角色的多样性。

Sequence assembly from 58 Malus WRKY ESTs resulted in 37 uniESTs. Malus zumi Mats is a salt-tolerant rootstock of apple, which could survive in 0.6% total salt content of land. Primers were designed in M. zumi Mats according to the 37 uniESTs, semi-quantitative RT-PCR was applied to reveal the expression patterns of MzWRKY gene family in response to salt stress. RT-PCR products of 28 MzWRKY genes were detectable, 21 MzWRKYs were up-regulated and 1 M_zWRKY was down regulated under salt stress. According to the peak hour, the MzWRKYs were classified into 2 groups. The different expression patterns of MzWRKY genes suggested that WRKY proteins play various roles in response to salt stress in M. zumi Mats.