沟叶结缕草胚性愈伤组织继代培养基对其生长和再生的影响

Effects of Subculture Media on Embryogenic Callus Growth and Regenera-tion of Zoysia matrella

以沟叶结缕草匍匐茎诱导的胚性愈伤组织为材料,通过连续3代的培养,比较了MS、N6和N6AA3种基本培养基与不同浓度(0.5、1.0、1.5、2.0mg·L-1)2,4-D组合对愈伤组织生长速度、胚性保持、再生能力、再生植株的抗氧化酶活性、脯氨酸含量和染色体倍性的影响。结果表明:MS培养基添加2.0mg·L-12,4-D对愈伤组织的生长和胚性保持效果最好,胚性愈伤组织比率达85.00%。但不同基本培养基对再生植株的SOD、POD、CAT活性和脯氨酸含量没有显著影响,流式细胞仪测定结果表明其染色体倍性也没有发生变化,植株个体间没有形态差异。

The embryogenic calluses induced from the stolons ofmanilagrass [Zoysia matrella （L.） Merr.] were taken as materials and cultivated on three basic media （MS, N6 and N6AA） with different concentrations （0.5, 1.0, 1.5 and 2.0 mg · L^-1 ） of2,4-D for three successive generations to compare their effects. Growth rate, embryogenic maintenance and regeneration ability of the calluses, activities of anti-oxidant enzymes, contents of proline and ploidy of the regenerated plants were measured. The results showed that MS medium supplemented with 2.0 mg · L^-1 2,4-D was optimal for callus growth and its embryogenic maintenance, and the rate of embryogenic callues reached 85.00%. However, there were no significant differences among the activities of SOD, POD, CAT and proline content in the regenerated plants acquired from the calluses that had been successively subcultured on three different basic media. The ploidy of the regenerated plants measured with flow cytometry showed unchanged. There were also no morphological differences appeared among individual plants regenerated from different treatments.