菊花营养生长期观赏性状的RAPD和ISSR标记

Detection of RAPD and ISSR Markers Associated with Ornamental Traits of Chrysanthemum in Vegetative Stage

以菊花‘雨花落英’和‘奥运含笑’及其142株F1群体为材料,采用单因素方差分析方法,检测了与F1群体营养生长期6个观赏性状关联的RAPD和ISSR遗传标记。结果表明,6个性状的观测值在F1群体中显著分离(变异系数大于20%),且均基本服从正态分布。单因素方差分析检测出与株高、株幅、株高/株幅比、节间长度、叶长和叶宽相关联遗传标记分别为15、9、9、11、7和8个,这些标记的累积贡献率均在30%以上,单个标记对目标性状的贡献率却较低(2.77%～8.50%),说明这些标记位点可能为微效多基因。与目标性状显著关联的标记位点的正确利用将进一步加速菊花选择育种的效率。

Association analysis were undertaken to detect the genetic markers significantly related to the 6 ornamental traits （i.e. plant height, plant width, the ratio of plant height to plant width, internode length, leaf length and leaf width） in vegetative stage by the method of One-Way ANOVA based on a F1 population derived from chrysanthemum cultivars, ＇Yuhualuoying＇ and ＇Aoyunhanxiao＇. The result showed that the 6 traits segregated significantly in the F1 population （coefficient of variation 〉 20%）, basically fitting in a normal distribution. The One-Way ANOVA analysis revealed that the number of genetic markers significantly associated with plant height, plant width, the ratio of plant height to plant width, internode length, leaf length and leaf width was 15, 9, 9, 11,7 and 8, respectively. Of these genetic markers, the cumulative contribution ratio for each trait was above 30%, whereas the contribution ratio of each single genetic marker ranged from 2.77% to 8.50%, revealing that these markers were polygene with little effect. We anticipated that the proper utilization of these genetic markers significantly associated with these target traits would effectively improve selection breeding in chrysanthemum.