摘要
目的探讨IL-10启动子基因多态性对乙肝肝硬化的影响。方法选择50例无肝硬化的慢性乙肝病毒携带者和42例肝炎后肝硬化患者为观察对象,采用限制性长片段多肽分析和直接测序的方法,检测IL-10启动子基因多态性,并将肝硬化组和无肝硬化组基因分布频率进行比较。结果无肝硬化组患者-592A分布频率明显高于肝硬化组;基因型-592A/A无肝硬化组明显高于硬化组;单倍体(-1082/-819/-592)ATA无硬化组频率明显高于硬化组。结论 IL-10启动子多态性对乙肝病毒感染后肝硬化的发展有一定的影响作用。
The present study is undertaken to investigate the association between live cirrhosis and interleukin-10 promoter polymorphisms in chronic hepatitis B patients.The sample population included 50 HBV carriers without live cirrhosis and 42 live cirrhosis patients with HBV infection.Three biallelic polymorphisms in the IL-10 gene promoter were analyzed by polymerase chain reaction in combination with direct sequencing or restriction fragment length polymorphism assay.The frequency of A allele at position-592 was higher in HBV carriers without live cirrhosis than that in live cirrhosis patients with HBV infection.In addition,the frequency of the IL-10 gene promoter-592A/A genotype,as well as the frequency of haplotypes in the IL-10 gene promoter （-1082/-819/592） ATA was higher in HBV carriers without live cirrhosis than in live cirrhosis patients with HBV infection.The results suggest that polymorphisms in the IL-10 promoter appear to have some influence on the development of fibrosis after HBV infection.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2010年第8期694-697,共4页
Immunological Journal
基金
福建省青年人才基金项目(2006F3102)
