大鼠8种肝脏细胞中缺血反应相关基因与肝再生的作用分析

The Function Analysis Between Ischemia Response-Related Genes In Eight Liver Cell Types And Rat Liver Regeneration

为了解8种肝脏细胞的缺血反应相关基因与大鼠肝再生的相关性,用percol密度梯度离心和免疫磁珠方法分离大鼠部分肝切除后不同时间(0,2,6,12,24,30,3672,120和168h)再生肝中的8种细胞,用Rat Genome 2302.0芯片等方法检测上述8种细胞的缺血反应相关基因在大鼠肝再生中表达变化,用生物学和系统生物学等方法分析上述基因与大鼠肝再生的相关性.结果显示,缺血反应主要在肝再生启动阶段及进展阶段前期发挥作用,且上调占优势,可刺激il6,tnf等肝再生关键基因表达;肝星形细胞、树突状细胞中的缺血反应相关基因具有表达的相似性.缺血反应能推动肝再生的顺利进行,但胆管上皮细胞的基因表达情况特殊,值得进一步研究.

For understanding the relationship between eight hepatic cells and rat liver regeneration, we separate the regenerating livers’ cells after partial hepatectomy （0, 2, 6, 12, 24, 30, 36, 72, 120 and 168 h） by percoll density gradient centrifugation and immunomagnetic selection, detect the ischemia reaction associated genes’ expression changes in the cells above by Rat Genome 230 2.0 chips, and analyze the relativity between these genes and rat liver regeneration by using biology and system biology. The results display that ischemia responses mainly play role at liver regeneration initial and progression phases, can stimulate the expression of liver regeneration key genes such as IL6 and TNF. The expression genes in BECs, HSCs and DCs have similarities. Ischemia response can push the liver regeneration smoothly, but BECs’ gene expression is different to other cells which need to research further.