辣椒中辣椒红素的分离及其含量测定

Study on the Separation of Capsanthin in Capsicum and Its Determination

目的:研究辣椒中辣椒红素的分离和含量测定方法。方法:采用高效液相色谱进行分离,并鉴定其成分及含量。色谱条件:InertsilOD-3色谱柱(4.6×250mm,5m),流动相:甲醇—二氯甲烷,流速:1ml/min,检测波长:474nm,柱温:30℃,进样量:10μl。结果:对辣椒红素提取液进行分离工艺探索,根据它的物理、化学特性对KOH用量、乙醚用量和静置时间进行了研究,经过二次旋转回归试验优化结合响应面分析,得出最高纯度和最优条件组合:KOH用量为5.39ml,乙醚用量为15.574ml,静置时间为2.34h,分离后的样品纯度由2.8%提高到17.2%,提高了6.14倍。结论:本研究在HPLC快速测定辣椒中辣椒红素中具有使用价值。

[Objective] To study the separation and determination of the capsanthin in capsicum. [Method] The capsanthin was separated and identified by high performance liquid chromatog-raphy （HPLC） using Inertsil OD-3 （4,6 × 250mm, 5/am）. The mobile phase was methanol-dichloromethane, total flow was lml/min, wavelength was 474nm, oven temperature was 30℃, injection volume was 10 μl. [Result] The purification of the extracted liquid of capsanthin was studied. According to its physical and chemical characteristic, the quantity of KOH, the quantity of ethyl ether and the standing time were studied. Meanwhile, the orthogonal and rotahle experimental design together with response surface graph analysis were also performed to get the upmost purity and optimal condition which was that the quantity of KOH was 5.39ml, the quantity of ether was 15.574ml, the standing time 2.34h. The purity of capanthin could reach 17.2%, which was approximately 6.14 times higher than the previous 2.8%. [Conclusion] This method could be used for rapid determination of capsanthin in capsicum with HPLC.