白假丝酵母菌耐药性及多药耐药基因CDR1和CDR2的表达

The resistance of Candida albicans to common antifungal drugs and its expression of CDR1 and CDR2 gene

目的:探讨女性下生殖道白假丝酵母菌耐药性及多药耐药基因CDR1和CDR2的表达。方法:培养、分离女性下生殖道假丝酵母菌感染耐药菌株,进行菌种鉴定、药敏试验和耐药性分析,应用RT-PCR检测多药耐药基因CDR1和CDR2的表达。结果:272例培养阳性的菌株中白假丝酵母菌、光滑假丝酵母菌、热带假丝酵母菌和克柔假丝酵母菌分别占79.8%、9.56%、8.10%和2.57%。217例白假丝酵母菌耐药菌株中特比耐芬耐药率最高,达94.93%,伊曲康唑和咪康唑的耐药率分别为15.21%和5.99%。RT-PCR显示白假丝酵母菌株耐药组CDR1和CDR2高表达。结论:女性下生殖道假丝酵母菌感染仍以白假丝酵母菌为主,非白假丝酵母菌感染呈上升趋势,白假丝酵母菌对常用抗真菌药物的耐药性呈上升趋势。白假丝酵母菌多药耐药基因CDR1和CDR2高表达,与临床白假丝酵母菌耐药密切相关。

Objective：To investigate the drug resistance in clinical Candida albicans strains and the expression of CDR1and CDR2 gene.Methods：There 176 Candida albicans were isolated and cultivated from various specimens and identify and drug susceptibility test was adopted.The drug resistance genes CDR1 /CDR2 were amplified by RT-PCR.Electrophoresis were used to check all PCR products for analysis of Candida albicans CDR1 and CDR2 genes.Results：From 167 strains of Candida,candida albicans,C.glabrata,C.tropicalis,C.krusei were 79.8 %,9.56 %,8.1 %,2.57 %,respectively.Of 217 Candida albicans strains,the resistant rates against tebinaifen was the highest,94.93%.The resistant rates against itroconazole and 5-flucytosine were 15.21% and 5.99%.The drug resistance genes CDR1 and CDR2 were overexpressed in Candida albicans resistant strains.Conclusion：The most common species in the clinical isolates are still Candida albicans,with non-albicans species appears increasing.The drug resistance of Candida albicans to commonly used antifungals shows a ascending trend.The incidences of CDR1 and CDR2 are overexpressed in Candida albicans resistant strains,which are close correlated with clinical Candida albicans resistance.