妊娠期糖尿病患者葡萄糖激酶基因多态性的实验研究

The experimental study on the glucokinase gene polymorphism in patients with gestational diabetes mellitus

目的:研究广东省汉族妊娠期糖尿病(GDM)孕妇葡萄糖激酶(GCK)基因多态性的分布频率及其在GDM发病中的作用。方法:选择80例GDM患者作为病例组,80例糖耐量正常的孕妇作为对照组,以稳态模型(Hom a model)公式评估胰岛素抵抗(IR),同时应用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)技术,检测GCK基因-259位点等位基因和基因型的分布频率。结果:①GDM组和对照组的血清FINS分别为(16.85±6.43)m IU/L和(25.16±11.95)m IU/L,两组比较有统计学差异(P<0.01);HOMA-IR分别为1.54±0.53和1.35±0.69,两组比较差异无统计学意义(P>0.05)。②GDM组GCK-259多态性位点T等位基因频率(0.127 6和0.034 5)及AT+TT基因型频率(0.2431和0.1153)显著高于对照组,差异有统计学意义(P<0.05)。结论:我国广东省汉族孕妇中,GCK基因-259位点A-T变异可增加孕妇患妊娠期糖尿病的危险性,A等位基因可能导致GDM的发病。

Objective：To investigate glucose gene polymorphism in patients with gestational diabetes mellitus in Hans of Guangdong provinceMethods：80 women with GDM and 80 health pregnancy women were studied.Fast serum insulin was measured by ELISA.IR was assessed by Homa model.Genotype of GCK was determined by PCR-RFLP.Results：①In GDM and NGT,the Serum FINS level was（16.85±6.43）mlU/L and（25.16±11.95）mIU/L（P〈0.01） respectively;HOMA-IR（1.54±0.53）and（1.35±0.69）（P〈0.05）respectively.②CCK gene A259T allele in GDM and control groups were 0.127 6 and 0.034 5,and frequencies of AT＋TT genotype were 0.2431 and 0.1153（P〈0.05）,respectively.Conclusion：In Hans of Guangdong province,CCK gene A259T mutation increases the risk of GDM.A allele may be involved in pathogenesis of GDM by decreasing the release of insulin.