一贯煎合四物汤对肝纤维化模型大鼠血清学指标的影响

Yiguan Decoction and Siwu Decoction on the Serological Indexes in Hepatic fibrosis Model Rats

目的观察一贯煎合四物汤对四氯化碳(CCl4)造模形成大鼠肝纤维化和肝硬化的保护作用。方法 60只大鼠随机分成6组,其中模型组和秋水仙碱组、一贯煎合四物汤高、中、低剂量组均采用改良CCl4法复制大鼠肝硬化模型,并从造模当日开始给药,另设正常对照组。造模44天后,检测各组血清HA、PCⅢ、IV-C含量,肝脏TGF-β1表达。结果一贯煎合四物汤各剂量组均可显著地降低动物血清HA、PCⅢ、Ⅳ-C的含量(均P<0.01),有效抑制肝组织TGF-β1的表达(均P<0.01)。结论一贯煎合四物汤可有效抑制肝纤维化。

Objective To observe Siwu Decoction and Yiguan Decoction on carbon tetrachloride induced liver fibrosis and cirrhosis in rats.Method 60 rats were randomly divided into six groups,among which the model group,colchicine group,Yiguan Decoction and Siwu Decoction high,medium,low dose groups were given medication after modeled by a modified CCl4 method.And,a normal control group was set.Rats in groups A to E were administered CCl4 for establishing animal model.44 days after the modeling,serum HA,PC Ⅲ,Ⅳ-C levels,expression of TGF-β1 in liver were observed.Results Yiguan Decoction and Siwu Decoction significantly reduced serum HA,PCⅢ,Ⅳ-C levels,inhibited hepatic expression of TGF-β1（P〈0.01 in all groups）.Conclusion Siwu Decoction and Yiguan Decoction effectively inhibit hepatic fibrosis.