用RT—PCR方法检测山东淄博地区人群庚型肝炎的感染状况

Investigation on the HGV Diagnosed by RT-PCR in the Population of Zibo Shandong Province

目的了解山东淄博地区人群庚型肝炎病毒感染状况。方法根据国外报道的庚型肝炎病毒基因组序列，设计了两对５’非编码区引物，用ＲＴ—ＰＣＲ方法检测了５３１例山东淄博地区甲、乙、丙、丁、戊型肝炎及献血员血清标本。结果不同人群ＨＧＶＲＮＡ阳性率分别为２２％，１４１％，２１１％，１１８％，１４％及６７％。结论山东淄博地区存在ＨＧＶ感染，并且与ＨＢＶ、ＨＣＶ合并感染较多，以经血传播为主。

Objective To investigate the infectious status of the HGV in the population of Zibo Shangdong. Methods Two sets of primers in noncoding region were designed according to the HGV genomic sequence reported by the foreign press. 531 cases of hepatitis A,B, C, D,E and blood doners were tested by RT-PCR. Results The positive rates of HGV RNA of the cases with hepatitis A,B,C,D,E and blood doners were 2.2%,14.1%,21.1%,11.8%,1.4% and 6.7% respectively. Conclusion The infection of HGV mostly co-existing with HBV and HCV could be found in Zibo of Shandong province which showed that the HGV is mainly transmitted by blood. [