外环境标本中检索致病性大肠菌方法的研究

Study on the Method to Isolate EPEC in the Environmental Samples

目的探讨提高外环境标本中致病性大肠菌分离培养阳性率的方法。方法采用特异吸附浓集法，以致病性大肠菌（ＥＰＥＣ）作为实验代表株进行了研究。结果ＯＫ多价抗血清致敏血球对标本中相对应的ＥＰＥＣ的吸附率为９１５％，二次洗涤对被检菌的丢失率为１０１％，杂菌的去除率为７２７％，方法的最低检出限比值为１／６４，比常规法高８倍。结论特异吸附浓集法很适合外环境标本的检索。

Objective To probe the method of increasing the positive isolation rate of EPEC in the environmental samples. Methods The specific absorption concentration method was used to study the EPEC. Results The absorption rate of OK polyvalent anti-serum sensitized blood cells to the relative EPEC in the sample was 91.5%. The losing rate of the tested bacteria after 2 times of washing was 10.1%. The removing rate of the mixed bacteria was 72.7%. The lowest detecting rate of this method was 1/64 which was 8 times higher than that of the routine method. Conclusion The specific absorption concentration method was suitable for the surveillance of the environmental samples. [