摘要
目的：建立卵巢健全乳腺小叶增生模型，评估Tamoxifen（TAM）对小鼠乳腺小叶增生的治疗效果。方法：用苯甲酸雌二醇（estradiol，E2）诱发卵巢健全小鼠乳腺小叶增生症，E2剂量为0．2mg/d肌肉注射，连续30d，以TAM0.5mg/d拌入饲料中，连续喂养30d。结果：模型组注射E222只小鼠均发生乳腺小叶增生病变，光镜下9只（41％）形态似人类单纯性小叶增生，6只（27％）形态似人类腺性小叶增生，5只（23％）形态似人类乳头状瘤病，2只（9％）形态似人类囊性小叶增生。其中上皮呈轻度增生12只（54％），高度增生5只（23％），不典型增生5只（23％）。预防组及治疗组病变明显减轻。免疫标记：兔抗雌二醇阳性率41％，兔抗孕酮阳性率90％。结论：E20.2mg/d连续用30d，可造成卵巢健全小鼠乳腺小叶增生的新模型，TAM具有明显抑制和治疗乳腺小叶增生的作用。
Objective:To establish a model of breast lobular hyperplasia in mice with normal ovaries and to evaluate the curative effect of tamoxifen on the animal model. Methods: Breast lobular hyperplasia was induced by estradiol(E2 0. 2 mg/d, im, for 30 days). Tamoxifen(TAM), an antiestrogen,was added to the feed in a dose of 0. 5 mg/d for 30 days. Results: The breast lobular hyperplasia was really induced in 22 mice injected with E2. By light microscopy, there were lesions similar to human simple lobular hyperplasia of breasts in 9 mice(41%), similar to human adenoid lobular hyperplasia in 6(27%), similar to human papillomatosis in 5(23%) and similar to human cystic hyperplasia in 2(9%). And there were mild, moderate and severe epithelial hyperplasia in 12(54%), 5(23%) and 5(23%), respectively. An alleviative effect on lobular hyperplasia was shown when TAM was preventively or therapeutically used.Immunolabelling method showed that the positive rates of antiestradiol and antiprogesterone of rabbit to experimental animal were 41% and 9%, respectively. Conclusion: The breast lobular hyperplasia in mice with normal ovaries can be well induced by E2 with a dose of 0.2 mg/d, for 30 days,and it is a modified animal model.TAM has markedly preventive and therapeutical effect on breast lobular hyperplasia in mice.
