α1肾上腺素受体亚型在人输尿管各段的分布特征研究

Distribution characterization of alpha1 adrenoreceptor subtypes in different segments of human ureter

目的采用形态学方法研究输尿管各段平滑肌束的特征及α1肾上腺素受体三种亚型的分布。方法选择行肾输尿管切除术或全膀胱切除术后无疾病累及的输尿管上中下段和膀胱壁间段,采用苏木素-伊红染色观察输尿管各段平滑肌束分布;并采用α1肾上腺素受体三种亚型特异性单克隆抗体SABC法定位输尿管各段α1肾上腺素受体亚型的分布。结果输尿管各段平滑肌以纵行肌束为主,靠近下段和输尿管膀胱壁间段移行为粗大纵行肌束,肾盂壁、肾盂输尿管连接部和输尿管各段均不存在典型环形肌束。结论人体输尿管各段以纵行平滑肌束为主。α1肾上腺素受体亚型α1A-AR、α1B-AR、α1D-AR在输尿管上中下段和膀胱壁间段均有分布,且愈靠近下段越丰富。

Objective To investigate the distribution characterization of smooth muscle bundle and alpha1 adrenoreceptor subtypes in different segments of human ureter.Methods Harvested the healthy ureteral segments including proximal/media/distal and ureterovesical segments from the surgical samples following operation.And measured the distribution of longitudinal and circular coursing muscle bundles of ureteral segments in hematoxylin and eosin staining and the alpha1 adrenoreceptor subtypes in different ureteral segments were examined by immunohistochemistry using α1 adrenoreceptor subtypes specific monoclonal antibodies.Results The microstructure of human ureter from proximal segment to ureterovesical segment showed the muscle bundles were so arranged that morphologically distinct longitudinal and circular layers cannot be clearly distinguished.Conclusion Our data suggests that the longitudinal smooth muscle bundles dominate in human ureter.The α1 adrenoreceptor subtypes α1A-AR,α1B-AR,α1D-AR mainly locate at the longitudinal muscle bundles of the ureter and the relative density of α1 adrenoreceptors are ureterovesical segmentdistal segmentmedia segmentproximal segment.