Autotaxin mRNA在人肝细胞癌中的表达

The expression of autotaxin mRNA in the human hepatocellular carcinoma

目的了解自分泌运动因子Ａｕｔｏｔａｘｉｎ（ＡＴＸ）ｍＲＮＡ在肝癌中的表达及其与肝癌临床病理特征之间的关系。方法应用半定量逆转录聚合酶链反应（ＲＴＰＣＲ）研究５例正常肝组织、３２例肝癌组织中ＡｕｔｏｔａｘｉｎｍＲＮＡ的表达，以βａｃｔｉｎ为内参照。结果５例正常肝组织及３２例肝癌中均有ＡｕｔｏｔａｘｉｎｍＲＮＡ表达；但Ａｕｔｏｔａｘｉｎ基因表达值（ｘ±ｓ）癌组织显著高于正常肝组织（６８．２３％±１５．３１％对３１．９７±８．０５％，Ｐ＜０．００１）。高表达ＡＴＸ肝癌与癌细胞肝内转移、门静脉癌栓形成、肿瘤的分化程度有关，而与年龄、性别、病因、肿瘤大小等无关。结论Ａｕｔｏｔａｘｉｎ基因过度表达与肝细胞癌的进展、转移有关。

Objective To investigate the expression of Autotaxin (ATX) mRNA in the human hepatocellular carcinoma(HCC) and whether there is correlation between ATX expression and clinicopathological features of HCC.Methods Five Specimens of normal liver tissues and thirty two histologically verified HCC specimens were studied. Semi quantitative reverse transcription polymerase chain reaction was used to detect mRNA expression of ATX. Results ATX was expressed in all 32 HCC and 5 normal liver tissues. The expression level of ATX gene in HCC samples was higher than that in normal liver tissues (68.23%±15.31% vs. 31.97% ±8.05%, P <0.001). Intrahepatic metastasis, vascular invasion and poor differentiation were more frequently noted in HCC patients with high ATX expression than in patients with low ATX expression.Conclusion The expression of ATX gene was related to HCC development and metastases.