凋膜止崩液对雌激素负荷去势大鼠P PR的影响

Effect of Diaomo Zhibeng Ye on Progesterone(P) Progesterone Receptors(PR) of Rats with Extra Estrogen and Ovariectomy

目的:研究不同剂量凋膜止崩液对雌激素负荷去势大鼠孕激素(P)及其受体(PR)的影响,揭示药物在有效部位靶向干预条件下,HPOA(下丘脑-垂体-卵巢轴)调节通道靶端药物效应机制,探讨靶向药物治疗功能失调性子宫出血(DUB)的分子生物学机制。方法:将大鼠随机分为4组:空白组(A)、模型组(B)、凋膜止崩液小剂量组(C)、凋膜止崩液大剂量组(D),采用雌激素负荷去势法建立大鼠增生过长子宫内膜动物模型,放射免疫法测定各组大鼠血清孕激素(P)水平,免疫组织化学染色观察大鼠子宫内膜细胞孕激素受体(PR)的表达。结果:血清中P的水平:B组与C组相比有显著性差异(P<0.01),C组与D组之间比较无统计学意义(P>0.05);子宫内膜PR的表达:C组、D组子宫内膜PR表达明显降低,与B组相比有显著统计学差异(P<0.01);C组与D组之间无明显统计学差异(P>0.05)。结论:凋膜止崩液可能通过直接或间接的作用调节P、PR消除增生过长子宫内膜。

Objective：To research the effect of the different dose medicine（Diaomo Zhibeng Ye）on the Progesterone（P）,Progesterone Receptors（PR）of rats with the extra estrogen and the ovariectomy,To reveal effective mechanism of Hypothalamus-Pituitary gland-Ovary Axis（HPOA）regulation channel on condition that is the effective part of drug of medical cure.To probe molecular biology mechanism on treatment of dysfunctional uterine bleeding.Methods：40 female SD rats were randomly divided into 4 group：Control group（A）,Modle-Making group（B）,Lower dosage group（C）,Higher dosage group（D）.Rats were to ovariectomy surgery,to set up an animal model-complex hyperplastic endometrial.The serum concentrations of progesterone were measured by radioimmunoassay（RIA）,Expression of Progesterone Receptor（PR）in endometrium cells were detected by immunohistoche.Results：The pregestrone level in serum：Compared with Group B and Group C,there is significant difference（P〈0.01）,there is statistical significance between Group B and Group D（P〉0.05）,there is no statistical significance between Group C and Group D（P〈0.05）.The expression of the PR in endometrium：PR of the Group C and Group D is obviously cut down compared with Group B,every group have significant difference（P〈0.01）;there is no statistical significance between Group C and Group D（P〈0.05）.Conclusion：Diaomo Zhibeng Ye could regulate P,PR through direct or indirect effect to refrain from Endometrial Hyperplasia.