腹安汤对腹泻型肠易激综合征大鼠血管活性肠肽和P物质的影响

Effects of Fu-an Decoction on Vasoactive Intestinal Peptide and Substance P in Rats of Diarrhea-Predominant Irritable Bowel Syndrome

目的:探讨腹安汤对腹泻型肠易激综合征大鼠的作用机制。方法:将48只大鼠随机分成正常组、模型组、腹安汤高、中、低剂量组及得舒特组;采用寒冷-束缚刺激法制备腹泻型肠易激综合征大鼠模型;观察给药前后大鼠的排便量,给药7天后的小肠推进功能,采用放射免疫法检测各组大鼠血浆VIP及SP含量的变化。结果:腹安汤高、中、低剂量组及得舒特组大鼠排便量减少、小肠推进运动减慢,与模型组比较差异有统计学意义(P<0.05);腹安汤高、中剂量组大鼠血浆VIP含量降低,与模型组比较有显著性差异(P<0.01);各组大鼠血浆SP含量变化均无统计学意义(P>0.05)。结论:腹安汤可抑制腹泻型肠易激综合征大鼠的小肠运动和血浆VIP的异常升高,可能是其发挥疗效的作用机制。

Objective：To explore the mechanism of Fu-a Decoction in the treatment of diarrhea-predominant irritable bowel syndrome（D-IBS）.Methods：48 Waster rats were divided randomly into 6 groups：normal group,model group,large-dose herb group,middle-dose herb group,small-dose herb group and Dicetel group.Cold-binding applications were given to establish similar model of D-IBS.Measuring the feces particles and the small intestinal propulsive function of the rats.Concentrations of gastrointestinal hormones（VIP,SP）in plasma were detected with RIA.Results：The low-dose group significantly reduce the feces particles and the small intestinal propulsive function,while the middle and large-dose herb group,the Dicetel group did it（P〈0.05）;The plasma VIP of high and middle dose group was reduced,compared with model group,there are significant differences（P〈0.01）;The plasma SP of various groups were not significant difference（P〉0.05）.Conclusion：Fu-an Decoction acts to treat D-IBS probably by adjusting the secretion of VIP and reducing the small intestinal propulsive function.