NaHCO_3胁迫下朝鲜接骨木和茶条槭苗木的生长及生理响应

Growth and Physiological Responses of Sambucus coreana and Acer ginnala Seedlings to NaHCO_3 Stress

采用盆栽试验的方法研究不同浓度NaHCO3(对照,1.0,2.0,4.0g·kg-1)胁迫下1年生朝鲜接骨木和茶条槭苗木的生长和生理生化指标的变化。结果显示:2树种苗木对低浓度的NaHCO3胁迫适应性较好,中、高浓度的NaHCO3胁迫下成活和生长受到明显抑制。在低、中、高处理下朝鲜接骨木存活率分别为100%,90%和65%,茶条槭存活率分别为100%,75%和55%。盐胁迫强度和时间均对2树种苗木的生理指标产生明显影响。随着盐胁迫浓度的增加和胁迫时间的延长,2树种苗木的MDA含量和细胞膜相对透性均显著升高,ASA和GSH含量呈上升趋势,脯氨酸和可溶性糖也大量积累。朝鲜接骨木在胁迫中期各处理GSH含量较对照分别增加68.5%,54.5%,90.4%,茶条槭在胁迫后期GSH含量较对照分别增加87.9%,124.9%,96.9%。朝鲜接骨木可溶性糖含量在胁迫后期各处理较对照分别增加46.7%,38.3%,51.9%;茶条槭分别增加15.6%,31.8%,22.7%。研究结果表明:2树种在低浓度盐胁迫环境下相关生理指标相对稳定,具有一定的抗性,可耐受的土壤NaHCO3的含量为1.0g·kg-1,朝鲜接骨木对碳酸盐的抗性略高于茶条槭。

The changes in growth,physiological and biochemical indexes of 1-year-old Sambucus coreana and Acer ginnala seedlings under different NaHCO3 stress intensities （CK,1. 0,2. 0,4. 0 g NaHCO3·kg -1 media） were tested. The two species able to adapt to the stress of low concentrations of NaHCO3,while the growth was dramatically inhibited under moderate and high concentrations of NaHCO3. The livability of Sambucus coreana were 100% ,90% and 65% respectively,in the low moderate and high treatments,and that of Acer ginnala was 100% ,75% and 55% respectively. There were remarkable intense and duration effects of the salt stress on physiogical indexes of the two species. By the increasing of stress concentration and duration of the stress time,the content of MDA and the relative osmosis of cell membrane significantly increased,and the contents of ASA and GSH also increased,and proline and soluble sugar contents were massively accumulated. Compared to the control,the content of GSH in Sambucus coreana under mild,moderate and severe stresses increased by 68. 5% ,54. 5% and 90. 4% at middle stress stage,while that of Acer ginnala increased by 87. 9% ,124. 9% and 96. 9% at long-time stress stage,respectively. Compared to the control,the contents of soluble sugar of Sambucus coreana and Acer ginnala at later stress stage increased by 46. 7% ,38. 3% ,51. 9% and 15. 6% ,31. 8% , 22. 7% ,respectively,under mild,moderate and severe stress. The results suggested that Sambucus coreana and Acer ginnala had tolerance to lower salt stress and could bear the soil environment with 1 g·kg -1 NaHCO3 . Sambucus coreana had stronger tolerance against carbonate stress than Acer ginnala.