乙型肝炎病毒(HBV)DNA免疫的初步研究

Primary Study of HBV DNA Immunization

用聚合酶链反应（ＰＣＲ）扩增了编码ＨＢＶｓＡｇ的基因序列，将其插入到ｐｃＤＮＡ３载体中，位于人巨细胞病毒（ＣＭＶ）早期启动子下游．重组质粒ｐｃＤＮＡ３－ｓＡｇ转染细胞后检测到ＨＢｓＡｇ表达．用纯化后的重组质粒直接注射到ＢＡＬＢ／Ｃ小鼠骨骼肌内，诱发实验小鼠产生了抗ＨＢｓＡｇ特异性抗体．

The HBsAg gene was amplified by polymerase chai n reaction (PCR) and cloned into a mammalian cell expressing vecter pcDNA3,under the control of the CMV promoter.The recombinant plasmid named pcDNA3 sAg was used to transfect He La cells.The results showed that transfected HeLa cells expressed HBsAg.After this,recombinant plasmid was injected directly into the skeletal muscles of BAL B/C mice,specific antibodes in sera were induced.PCR detection demonstrated that HBsAg gene was not integrated into the host chromosome.