HIV-1和HBV复合型DNA免疫的初步研究

HIV-1 and HBV Chimeric DNA Immunization

近年的研究表明，在啮齿类和非人灵长类免疫带有编码病毒和细菌抗原基因的质粒ＤＮＡ可以激发体液和细胞免疫应答．在本实验中，将ＨＢＶ的Ｓ基因和ＨＩＶ－１的ｇｐ１６０基因以融合形式插入到载体ｐｃＤＮＡ３中，其能表达ＨＢｓＡｇ和ｇｐ１６０的融合蛋白，并将此质粒ＤＮＡ分别直接注射到Ｂａｌｂ／ｃ小鼠和Ｓｗｉｓ小鼠．三次免疫后，用ＥＬＩＳＡ的方法初步检测ＨＢｓＡｇ和ｇｐ１６０抗原特异的抗体免疫应答均为阳性．结果说明，带有ＨＢＶ和ＨＩＶ－１融合基因的质粒ＤＮＡ直接免疫小鼠后，均激发了小鼠产生相应的免疫应答反应。

The recent studies support that immunization with plasmid DNA encodi ng genes for viral or bacterial antigens can elicit both humoral and cellular im mune in rodents and non human primates.In this study,Balb/c and Swiss mice we re vaccinated by injection with plasmids, which S gene of HBV and gp160 gene of HIV-1 were cloned into pcDNA3,it can express HBsAg of HBV and gp160 of HIV-1 in fusion form.Antigen specific antibody responses against HBsAg and gp160 protei ns were measured by ELISA.It was concluded that antibody titers against HBsAg a nd gp160 are positive after three immunizations.Antibodies' responses demonstra ted that pcDNA3 cloned with fusion form of S gene and gp160 gene can elicit humo ral immunities respectively.This result provides us a new method to research an d produce multi covalent vaccine.